Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

BAG6 is a novel microtubule-binding protein that regulates ciliogenesis by modulating the cell cycle and interacting with ?-tubulin.

He X, Zhang Y, Yang L, Feng J, Yang S, Li T, Zhong T, Li Q, Xie W, Liu M, Zhou J, Li D, Xie S

Microtubule-binding proteins provide an alternative and vital pathway to the functional diversity of microtubules. Considerable work is still required to understand the complexities of microtubule-associated cellular processes and to identify novel microtubule-binding proteins. In this study, we identify Bcl2-associated athanogene cochaperone 6 (BAG6) as a novel microtubule-binding protein and reveal that it is crucial for primary ciliogenesis. By immunofluorescence we ... [more]

Exp Cell Res Dec. 01, 2019; 387(1);111776 [Pubmed: 31838060]

Throughput

  • Low Throughput

Curated By

  • BioGRID