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BAIT

INSIG1

CL-6, CL6

insulin induced gene 1

GO Process (5)

GO Function (1)

GO Component (3)

Gene Ontology Biological Process

SREBP signaling pathway [IDA]

cell proliferation [TAS]

metabolic process [TAS]

negative regulation of cargo loading into COPII-coated vesicle [IMP]

small molecule metabolic process [TAS]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

SREBP-SCAP-Insig complex [IDA]

endoplasmic reticulum [IDA]

endoplasmic reticulum membrane [TAS]

CRISPR Database OMIM VEGA HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

EEF1A1

CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, GRAF-1EF, HNGC:16303, LENG7, PTI1, eEF1A-1, RP11-505P4.2

eukaryotic translation elongation factor 1 alpha 1

GO Process (5)

GO Function (5)

GO Component (8)

Gene Ontology Biological Process

cellular protein metabolic process [TAS]

cellular response to epidermal growth factor stimulus [IDA]

gene expression [TAS]

translation [TAS]

translational elongation [TAS]

Gene Ontology Molecular Function

GTP binding [TAS]
poly(A) RNA binding [IDA]
protein binding [IPI]
protein kinase binding [IPI]
translation elongation factor activity [TAS]

Gene Ontology Cellular Component

cytoplasm [IDA, ISS, TAS]

eukaryotic translation elongation factor 1 complex [TAS]

extracellular space [IDA]

extracellular vesicular exosome [IDA]

nucleolus [IDA]

CRISPR Database HGNC Alliance of Genome Resources OMIM Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

Xu D, Wang Z, Xia Y, Shao F, Xia W, Wei Y, Li X, Qian X, Lee JH, Du L, Zheng Y, Lv G, Leu JS, Wang H, Xing D, Liang T, Hung MC, Lu Z

Cancer cells increase lipogenesis for their proliferation and the activation of sterol regulatory element-binding proteins (SREBPs) has a central role in this process. SREBPs are inhibited by a complex composed of INSIG proteins, SREBP cleavage-activating protein (SCAP) and sterols in the endoplasmic reticulum. Regulation of the interaction between INSIG proteins and SCAP by sterol levels is critical for the dissociation ... [more]

Nature Dec. 01, 2019; 580(7804);530-535 [Pubmed: 32322062]

Throughput

High Throughput

Curated By

BioGRID