BAIT

INSIG1

CL-6, CL6

insulin induced gene 1

GO Process (5)

GO Function (1)

GO Component (3)

Gene Ontology Biological Process

SREBP signaling pathway [IDA]

cell proliferation [TAS]

metabolic process [TAS]

negative regulation of cargo loading into COPII-coated vesicle [IMP]

small molecule metabolic process [TAS]

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

SREBP-SCAP-Insig complex [IDA]

endoplasmic reticulum [IDA]

endoplasmic reticulum membrane [TAS]

CRISPR Database OMIM VEGA HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

PREY

TFRC

CD71, T9, TFR, TFR1, TR, TRFR, p90

transferrin receptor

Synthetic Protein Interaction Project

GO Process (6)

GO Function (5)

GO Component (14)

Gene Ontology Biological Process

cellular iron ion homeostasis [NAS, TAS]

iron ion import [IDA]

regulation of cell growth [IMP]

regulation of cell proliferation [IMP]

transferrin transport [TAS]

transmembrane transport [TAS]

Gene Ontology Molecular Function

double-stranded RNA binding [IDA]
identical protein binding [IPI]
poly(A) RNA binding [IDA]
protein binding [IPI]
transferrin receptor activity [NAS]

Gene Ontology Cellular Component

blood microparticle [IDA]

cell surface [IDA]

coated pit [IDA]

cytoplasmic membrane-bounded vesicle [IDA]

extracellular region [IDA]

extracellular space [IDA]

extracellular vesicular exosome [IDA]

integral component of plasma membrane [TAS]

intracellular membrane-bounded organelle [IDA]

plasma membrane [TAS]

recycling endosome [IDA]

CRISPR Database OMIM VEGA HGNC Alliance of Genome Resources Entrez Gene RefSeq UniprotKB Ensembl HPRD

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.

Xu D, Wang Z, Xia Y, Shao F, Xia W, Wei Y, Li X, Qian X, Lee JH, Du L, Zheng Y, Lv G, Leu JS, Wang H, Xing D, Liang T, Hung MC, Lu Z

Cancer cells increase lipogenesis for their proliferation and the activation of sterol regulatory element-binding proteins (SREBPs) has a central role in this process. SREBPs are inhibited by a complex composed of INSIG proteins, SREBP cleavage-activating protein (SCAP) and sterols in the endoplasmic reticulum. Regulation of the interaction between INSIG proteins and SCAP by sterol levels is critical for the dissociation ... [more]

Nature Dec. 01, 2019; 580(7804);530-535 [Pubmed: 32322062]

Throughput

High Throughput

Curated By

BioGRID