BAIT

AGO2

EIF2C2, Q10
argonaute RISC catalytic component 2
Homo sapiens

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

CircRNA cRAPGEF5 inhibits the growth and metastasis of renal cell carcinoma via the miR-27a-3p/TXNIP pathway.

Chen Q, Liu T, Bao Y, Zhao T, Wang J, Wang H, Wang A, Gan X, Wu Z, Wang L

Circular RNAs (circRNAs) are reported to act as important regulators in cancers. CircRNA RAPGEF5 (cRAPGEF5) is derived from exons 2-6 of the RAPGEF5 gene and may promote papillary thyroid cancer progression. However, the role of cRAPGEF5 in renal cell carcinoma (RCC) remains unclear. In this study, we found cRAPGEF5 to be significantly downregulated in RCC tissues. Among 245 RCC cases, ... [more]

Cancer Lett Dec. 28, 2019; 469();68-77 [Pubmed: 31629934]

Throughput

  • Low Throughput

Additional Notes

  • assayed using RIP (RNA immunoprecipitation) in which protein corresponding to the bait gene AGO2 was IPd and associated circular RNA corresponding to the hit/prey gene RAPGEF5 was detected by RT-qPCR (reverse transcription quantitative polymerase chain reaction) and agarose gel electrophoresis

Curated By

  • BioGRID