BAIT
NSMF
HH9, NELF, RP11-48C7.1
NMDA receptor synaptonuclear signaling and neuronal migration factor
GO Process (8)
GO Function (1)
GO Component (15)
Gene Ontology Biological Process
- cellular response to amino acid stimulus [ISS]
- cellular response to electrical stimulus [ISS]
- cellular response to gonadotropin stimulus [ISS]
- positive regulation of neuron migration [IMP]
- positive regulation of protein dephosphorylation [ISS]
- regulation of dendrite morphogenesis [ISS]
- regulation of neuron apoptotic process [ISS]
- regulation of neuronal synaptic plasticity [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
IL1A
IL-1A, IL1, IL1-ALPHA, IL1F1
interleukin 1, alpha
GO Process (16)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- apoptotic process [TAS]
- cell proliferation [TAS]
- cellular response to heat [IDA]
- cytokine-mediated signaling pathway [IMP]
- inflammatory response [TAS]
- negative regulation of cell proliferation [IDA]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [TAS]
- positive regulation of ERK1 and ERK2 cascade [IBA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IBA]
- positive regulation of JNK cascade [IBA]
- positive regulation of angiogenesis [ISS]
- positive regulation of cytokine secretion [IDA]
- positive regulation of interleukin-2 biosynthetic process [IMP]
- positive regulation of mitosis [IMP]
- positive regulation vascular endothelial growth factor production [ISS]
- response to copper ion [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-RNA
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.
Publication
IL-1 Transcriptional Responses to Lipopolysaccharides Are Regulated by a Complex of RNA Binding Proteins.
The IL1A and IL1B genes lie in close proximity on chromosome 2 near the gene for their natural inhibitor, IL1RN Despite diverse functions, they are all three inducible through TLR4 signaling but with distinct kinetics. This study analyzed transcriptional induction kinetics, chromosome looping, and enhancer RNA production to understand the distinct regulation of these three genes in human cells. IL1A, ... [more]
J Immunol Dec. 01, 2019; 204(5);1334-1344 [Pubmed: 31953354]
Throughput
- Low Throughput
Additional Notes
- assayed using RIP (RNA immunoprecipitation) in which protein corresponding to bait gene NELF was IPd and associated enhancer-derived RNA corresponding to prey/hit genes IL1A and IL1B was detected by qRT-PCR (quantitative reverse transcription polymerase chain reaction)
Curated By
- BioGRID