An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Tumor-derived neomorphic mutations in ASXL1 impairs the BAP1-ASXL1-FOXK1/K2 transcription network.

Xia YK, Zeng YR, Zhang ML, Liu P, Liu F, Zhang H, He CX, Sun YP, Zhang JY, Zhang C, Song L, Ding C, Tang YJ, Yang Z, Yang C, Wang P, Guan KL, Xiong Y, Ye D

Additional sex combs-like 1 (ASXL1) interacts with BRCA1-associated protein 1 (BAP1) deubiquitinase to oppose the polycomb repressive complex 1 (PRC1)-mediated histone H2A ubiquitylation. Germline BAP1 mutations are found in a spectrum of human malignancies, while ASXL1 mutations recurrently occur in myeloid neoplasm and are associated with poor prognosis. Nearly all ASXL1 mutations are heterozygous frameshift or nonsense mutations in the ... [more]

Protein Cell Jul. 18, 2020; (); [Pubmed: 32683582]

Throughput

High Throughput

Additional Notes

Interact with full-length ASXL

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ASXL1 PHGDH	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Tsuboyama N (2022)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ASXL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

peroxisome proliferator activated receptor binding [ISS]protein binding [IPI]retinoic acid receptor binding [ISS]transcription coactivator activity [ISS]transcription corepressor activity [ISS]

Gene Ontology Cellular Component

PHGDH

Gene Ontology Biological Process

Gene Ontology Molecular Function

electron carrier activity [TAS]phosphoglycerate dehydrogenase activity [TAS]