BAIT

DNAJA4

MST104, MSTP104, PRO1472
DnaJ (Hsp40) homolog, subfamily A, member 4
GO Process (2)
GO Function (3)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

TUBB6

HsT1601, TUBB-5
tubulin, beta 6 class V
GO Process (3)
GO Function (0)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains.

Piette BL, Alerasool N, Lin ZY, Lacoste J, Lam MHY, Qian WW, Tran S, Larsen B, Campos E, Peng J, Gingras AC, Taipale M

Hsp70s comprise a deeply conserved chaperone family that has a central role in maintaining protein homeostasis. In humans, Hsp70 client specificity is provided by 49 different co-factors known as J domain proteins (JDPs). However, the cellular function and client specificity of JDPs have largely remained elusive. We have combined affinity purification-mass spectrometry (AP-MS) and proximity-dependent biotinylation (BioID) to characterize the ... [more]

Mol Cell Apr. 27, 2021; (); [Pubmed: 33957083]

Quantitative Score

  • 3.55 [FoldChange]

Throughput

  • High Throughput

Additional Notes

  • High confidence interactions were identified as having an average probability of the identified interaction (AvgP) >= 0.98. The associated score represents the fold change of spectral counts (or intensities) for each individual interaction.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
DNAJA4 TUBB6
Affinity Capture-Luminescence
Affinity Capture-Luminescence

An interaction is inferred when a bait protein, tagged with luciferase, is enzymatically detected in immunoprecipitates of the prey protein as light emission. The prey protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag.

Low-BioGRID
-
DNAJA4 TUBB6
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-

Curated By

  • BioGRID