An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains.

Piette BL, Alerasool N, Lin ZY, Lacoste J, Lam MHY, Qian WW, Tran S, Larsen B, Campos E, Peng J, Gingras AC, Taipale M

Hsp70s comprise a deeply conserved chaperone family that has a central role in maintaining protein homeostasis. In humans, Hsp70 client specificity is provided by 49 different co-factors known as J domain proteins (JDPs). However, the cellular function and client specificity of JDPs have largely remained elusive. We have combined affinity purification-mass spectrometry (AP-MS) and proximity-dependent biotinylation (BioID) to characterize the ... [more]

Mol Cell Apr. 27, 2021; (); [Pubmed: 33957083]

Quantitative Score

8.57 [FoldChange]

Throughput

High Throughput

Additional Notes

High confidence interactions were identified as having an average probability of the identified interaction (AvgP) >= 0.98. The associated score represents the fold change of spectral counts (or intensities) for each individual interaction.

Curated By

BioGRID

Interaction Summary

DNAJC2

Gene Ontology Biological Process

Gene Ontology Molecular Function

Hsp70 protein binding [IPI]chromatin binding [IDA]histone binding [IDA]poly(A) RNA binding [IDA]ubiquitin binding [IDA]

Gene Ontology Cellular Component

RPL9

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA binding [TAS]structural constituent of ribosome [NAS]