SEC63
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
VAPA
Gene Ontology Biological Process
- COPII-coated vesicle budding [IMP]
- ER to Golgi vesicle-mediated transport [IMP]
- cell death [IMP]
- endoplasmic reticulum organization [IMP]
- membrane fusion [TAS]
- negative regulation by host of viral genome replication [IDA]
- neuron projection development [IMP]
- positive regulation by host of viral genome replication [IDA]
- positive regulation by host of viral release from host cell [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- protein folding in endoplasmic reticulum [IMP]
- protein localization to endoplasmic reticulum [IMP]
- signal transduction [IMP]
- small molecule metabolic process [TAS]
- sphingolipid biosynthetic process [TAS]
- sphingolipid metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains.
Hsp70s comprise a deeply conserved chaperone family that has a central role in maintaining protein homeostasis. In humans, Hsp70 client specificity is provided by 49 different co-factors known as J domain proteins (JDPs). However, the cellular function and client specificity of JDPs have largely remained elusive. We have combined affinity purification-mass spectrometry (AP-MS) and proximity-dependent biotinylation (BioID) to characterize the ... [more]
Quantitative Score
- 540.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- High confidence interactions were identified as having an average probability of the identified interaction (AvgP) >= 0.98. The associated score represents the fold change of spectral counts (or intensities) for each individual interaction.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| VAPA SEC63 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3374755 | |
| VAPA SEC63 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 81.67 | BioGRID | 3010292 | |
| SEC63 VAPA | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | 36.49 | BioGRID | 2940735 |
Curated By
- BioGRID