BAIT
DNAJB2
DSMA5, HSJ-1, HSJ1, HSPF3
DnaJ (Hsp40) homolog, subfamily B, member 2
GO Process (10)
GO Function (6)
GO Component (4)
Gene Ontology Biological Process
- ER-associated ubiquitin-dependent protein catabolic process [IDA]
- negative regulation of cell growth [IGI]
- negative regulation of cell proliferation [IGI]
- negative regulation of inclusion body assembly [IDA]
- negative regulation of protein deubiquitination [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein ubiquitination [IDA]
- protein folding [TAS]
- protein refolding [IDA]
- response to unfolded protein [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
SNX6
MSTP010, TFAF2
sorting nexin 6
GO Process (8)
GO Function (4)
GO Component (6)
Gene Ontology Biological Process
- endocytosis [IBA]
- intracellular protein transport [IMP, NAS]
- negative regulation of epidermal growth factor-activated receptor activity [NAS]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [IDA]
- regulation of histamine secretion by mast cell [IMP]
- retrograde transport, endosome to Golgi [IMP]
- vesicle organization [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains.
Hsp70s comprise a deeply conserved chaperone family that has a central role in maintaining protein homeostasis. In humans, Hsp70 client specificity is provided by 49 different co-factors known as J domain proteins (JDPs). However, the cellular function and client specificity of JDPs have largely remained elusive. We have combined affinity purification-mass spectrometry (AP-MS) and proximity-dependent biotinylation (BioID) to characterize the ... [more]
Mol Cell Apr. 27, 2021; (); [Pubmed: 33957083]
Quantitative Score
- 65.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- High confidence proximal protein interactions had an average probability of the identified interaction (AvgP) >= 0.98. The associated score represents the fold change of spectral counts (or intensities) for each individual interaction.
Curated By
- BioGRID