BAIT
DNAJC10
ERdj5, JPDI, MTHr, PDIA19, UNQ495/PRO1012
DnaJ (Hsp40) homolog, subfamily C, member 10
GO Process (6)
GO Function (9)
GO Component (4)
Gene Ontology Biological Process
- ER-associated ubiquitin-dependent protein catabolic process [IMP]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [IDA]
- negative regulation of protein phosphorylation [IDA]
- positive regulation of ATPase activity [ISS]
- protein folding in endoplasmic reticulum [IDA]
- response to endoplasmic reticulum stress [IDA]
Gene Ontology Molecular Function- ATPase activator activity [ISS]
- ATPase binding [IPI]
- Hsp70 protein binding [IPI]
- chaperone binding [IDA]
- disulfide oxidoreductase activity [ISS]
- misfolded protein binding [IDA]
- oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor [ISS]
- protein binding [IPI]
- protein disulfide oxidoreductase activity [IDA, ISS]
- ATPase activator activity [ISS]
- ATPase binding [IPI]
- Hsp70 protein binding [IPI]
- chaperone binding [IDA]
- disulfide oxidoreductase activity [ISS]
- misfolded protein binding [IDA]
- oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor [ISS]
- protein binding [IPI]
- protein disulfide oxidoreductase activity [IDA, ISS]
Gene Ontology Cellular Component
Homo sapiens
PREY
CKAP4
CLIMP-63, ERGIC-63, p63
cytoskeleton-associated protein 4
GO Process (0)
GO Function (1)
GO Component (3)
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains.
Hsp70s comprise a deeply conserved chaperone family that has a central role in maintaining protein homeostasis. In humans, Hsp70 client specificity is provided by 49 different co-factors known as J domain proteins (JDPs). However, the cellular function and client specificity of JDPs have largely remained elusive. We have combined affinity purification-mass spectrometry (AP-MS) and proximity-dependent biotinylation (BioID) to characterize the ... [more]
Mol Cell Apr. 27, 2021; (); [Pubmed: 33957083]
Quantitative Score
- 8.82 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- High confidence proximal protein interactions had an average probability of the identified interaction (AvgP) >= 0.98. The associated score represents the fold change of spectral counts (or intensities) for each individual interaction.
Curated By
- BioGRID