BAIT
DNAJC25
bA16L21.2.1
DnaJ (Hsp40) homolog, subfamily C , member 25
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
RAB3GAP1
P130, RAB3GAP, RAB3GAP130, WARBM1
RAB3 GTPase activating protein subunit 1 (catalytic)
GO Process (14)
GO Function (3)
GO Component (4)
Gene Ontology Biological Process
- brain development [IMP]
- camera-type eye development [IMP]
- establishment of protein localization to endoplasmic reticulum membrane [IMP]
- face morphogenesis [IMP]
- hypothalamus development [IMP]
- lipid particle organization [IMP]
- positive regulation of GTP catabolic process [IMP]
- positive regulation of Rab GTPase activity [IMP, ISS]
- positive regulation of endoplasmic reticulum tubular network organization [IMP]
- positive regulation of glutamate neurotransmitter secretion in response to membrane depolarization [ISS]
- regulation of GTPase activity [IDA]
- regulation of calcium ion-dependent exocytosis of neurotransmitter [ISS]
- regulation of excitatory postsynaptic membrane potential [ISS]
- regulation of short-term neuronal synaptic plasticity [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains.
Hsp70s comprise a deeply conserved chaperone family that has a central role in maintaining protein homeostasis. In humans, Hsp70 client specificity is provided by 49 different co-factors known as J domain proteins (JDPs). However, the cellular function and client specificity of JDPs have largely remained elusive. We have combined affinity purification-mass spectrometry (AP-MS) and proximity-dependent biotinylation (BioID) to characterize the ... [more]
Mol Cell Apr. 27, 2021; (); [Pubmed: 33957083]
Quantitative Score
- 250.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- High confidence proximal protein interactions had an average probability of the identified interaction (AvgP) >= 0.98. The associated score represents the fold change of spectral counts (or intensities) for each individual interaction.
Curated By
- BioGRID