BAIT
DNAJC25
bA16L21.2.1
DnaJ (Hsp40) homolog, subfamily C , member 25
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
EXT2
SOTV
exostosin glycosyltransferase 2
GO Process (9)
GO Function (7)
GO Component (6)
Gene Ontology Biological Process
- carbohydrate metabolic process [TAS]
- cellular polysaccharide biosynthetic process [IDA]
- glycosaminoglycan biosynthetic process [IDA, TAS]
- glycosaminoglycan metabolic process [TAS]
- heparan sulfate proteoglycan biosynthetic process [IMP]
- heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process [IMP]
- ossification [IMP]
- signal transduction [TAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function- N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity [NAS]
- acetylglucosaminyltransferase activity [IDA]
- glucuronosyltransferase activity [IDA]
- heparan sulfate N-acetylglucosaminyltransferase activity [NAS]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA]
- transferase activity, transferring glycosyl groups [IDA]
- N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity [NAS]
- acetylglucosaminyltransferase activity [IDA]
- glucuronosyltransferase activity [IDA]
- heparan sulfate N-acetylglucosaminyltransferase activity [NAS]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA]
- transferase activity, transferring glycosyl groups [IDA]
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Comprehensive interactome profiling of the human Hsp70 network highlights functional differentiation of J domains.
Hsp70s comprise a deeply conserved chaperone family that has a central role in maintaining protein homeostasis. In humans, Hsp70 client specificity is provided by 49 different co-factors known as J domain proteins (JDPs). However, the cellular function and client specificity of JDPs have largely remained elusive. We have combined affinity purification-mass spectrometry (AP-MS) and proximity-dependent biotinylation (BioID) to characterize the ... [more]
Mol Cell Apr. 27, 2021; (); [Pubmed: 33957083]
Quantitative Score
- 60.0 [FoldChange]
Throughput
- High Throughput
Additional Notes
- BioID
- High confidence proximal protein interactions had an average probability of the identified interaction (AvgP) >= 0.98. The associated score represents the fold change of spectral counts (or intensities) for each individual interaction.
Curated By
- BioGRID