MBD2
Gene Ontology Biological Process
Gene Ontology Molecular Function- C2H2 zinc finger domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- methyl-CpG binding [IDA, NAS]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- satellite DNA binding [TAS]
- C2H2 zinc finger domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- methyl-CpG binding [IDA, NAS]
- nucleosomal DNA binding [IDA]
- protein binding [IPI]
- protein domain specific binding [IPI]
- satellite DNA binding [TAS]
Gene Ontology Cellular Component
HDAC2
Gene Ontology Biological Process
- ATP-dependent chromatin remodeling [IDA]
- blood coagulation [TAS]
- chromatin remodeling [IC]
- circadian regulation of gene expression [ISS]
- dendrite development [ISS]
- embryonic digit morphogenesis [ISS]
- epidermal cell differentiation [ISS]
- eyelid development in camera-type eye [ISS]
- fungiform papilla formation [ISS]
- hair follicle placode formation [ISS]
- histone H3 deacetylation [ISS]
- histone H4 deacetylation [ISS]
- histone deacetylation [IMP]
- maintenance of chromatin silencing [IMP]
- negative regulation of MHC class II biosynthetic process [IC]
- negative regulation of apoptotic process [ISS]
- negative regulation of cell cycle [TAS]
- negative regulation of neuron projection development [ISS]
- negative regulation of sequence-specific DNA binding transcription factor activity [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [IC, IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- odontogenesis of dentin-containing tooth [ISS]
- positive regulation of cell proliferation [IMP]
- positive regulation of collagen biosynthetic process [IC]
- positive regulation of proteolysis [IMP]
- positive regulation of receptor biosynthetic process [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- positive regulation of transcription, DNA-templated [IC]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II repressing transcription factor binding [IPI]
- chromatin binding [ISS]
- deacetylase activity [ISS]
- enzyme binding [IPI]
- histone deacetylase activity [IDA]
- nucleosomal DNA binding [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein deacetylase activity [IMP]
- sequence-specific DNA binding [IDA]
- transcription factor binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II distal enhancer sequence-specific DNA binding [IDA]
- RNA polymerase II repressing transcription factor binding [IPI]
- chromatin binding [ISS]
- deacetylase activity [ISS]
- enzyme binding [IPI]
- histone deacetylase activity [IDA]
- nucleosomal DNA binding [IDA]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- protein deacetylase activity [IMP]
- sequence-specific DNA binding [IDA]
- transcription factor binding [IPI]
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]
Quantitative Score
- 27.98329353 [HGSCore]
Throughput
- High Throughput
Additional Notes
- Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| HDAC2 MBD2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| HDAC2 MBD2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 3357451 | |
| MBD2 HDAC2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 0.9905 | BioGRID | 3271485 | |
| HDAC2 MBD2 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1 | BioGRID | 871957 | |
| MBD2 HDAC2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| MBD2 HDAC2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| MBD2 HDAC2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| MBD2 HDAC2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| HDAC2 MBD2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 3453161 | |
| MBD2 HDAC2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.4917 | BioGRID | 1262929 | |
| MBD2 HDAC2 | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID