BAIT

UQCRFS1

RIP1, RIS1, RISP, UQCR5
ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
GO Process (3)
GO Function (0)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens
PREY

CIAO1

CIA1, WDR39
cytosolic iron-sulfur assembly component 1
UPS Project
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.

Marcon E, Ni Z, Pu S, Turinsky AL, Trimble SS, Olsen JB, Silverman-Gavrila R, Silverman-Gavrila L, Phanse S, Guo H, Zhong G, Guo X, Young P, Bailey S, Roudeva D, Zhao D, Hewel J, Li J, Graeslund S, Paduch M, Kossiakoff AA, Lupien M, Emili A, Wodak SJ, Greenblatt J

Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]

Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]

Quantitative Score

  • 24.64239307 [HGSCore]

Throughput

  • High Throughput

Additional Notes

  • Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11

Curated By

  • BioGRID