BAIT
SETD8
KMT5A, PR-Set7, SET07, SET8
SET domain containing (lysine methyltransferase) 8
GO Process (6)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- histone lysine methylation [IDA]
- mitotic cell cycle [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- peptidyl-lysine monomethylation [IDA]
- regulation of DNA damage response, signal transduction by p53 class mediator [IMP]
Gene Ontology Molecular Function
Homo sapiens
PREY
RPL10A
CSA19, Csa-19, L10A, NEDD6
ribosomal protein L10a
GO Process (14)
GO Function (3)
GO Component (8)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- SRP-dependent cotranslational protein targeting to membrane [TAS]
- anatomical structure morphogenesis [TAS]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- translation [NAS, TAS]
- translational elongation [TAS]
- translational initiation [TAS]
- translational termination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral transcription [TAS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]
Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]
Quantitative Score
- 24.63429915 [HGSCore]
Throughput
- High Throughput
Additional Notes
- Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11
Curated By
- BioGRID