BAIT
SUPT4H1
SPT4, SPT4H, SUPT4H
suppressor of Ty 4 homolog 1 (S. cerevisiae)
GO Process (12)
GO Function (3)
GO Component (3)
Gene Ontology Biological Process
- chromatin remodeling [TAS]
- gene expression [TAS]
- negative regulation of DNA-templated transcription, elongation [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of DNA-templated transcription, elongation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of viral transcription [TAS]
- regulation of transcription from RNA polymerase II promoter [TAS]
- regulation of transcription, DNA-templated [TAS]
- transcription elongation from RNA polymerase II promoter [IDA, TAS]
- transcription from RNA polymerase II promoter [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- DSIF complex [IDA]
- nucleoplasm [IDA, TAS]
- nucleus [IDA]
Homo sapiens
PREY
RNF34
CARP-1, CARP1, RFI, RIF, RIFF, hRFI
ring finger protein 34, E3 ubiquitin protein ligase
GO Process (9)
GO Function (5)
GO Component (3)
Gene Ontology Biological Process
- negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis [IMP]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [IMP]
- negative regulation of signal transduction by p53 class mediator [IMP]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA, IMP]
- protein K48-linked ubiquitination [IMP]
- protein ubiquitination [IDA, IMP]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- regulation of oxygen metabolic process [ISS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]
Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]
Quantitative Score
- 20.83985018 [HGSCore]
Throughput
- High Throughput
Additional Notes
- Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11
Curated By
- BioGRID