BAIT
NDC80
HEC, HEC1, HsHec1, KNTC2, TID3, hsNDC80
NDC80 kinetochore complex component
GO Process (8)
GO Function (2)
GO Component (8)
Gene Ontology Biological Process
- attachment of mitotic spindle microtubules to kinetochore [IBA]
- attachment of spindle microtubules to kinetochore [IMP]
- chromosome segregation [IMP]
- establishment of mitotic spindle orientation [IMP]
- mitotic cell cycle [TAS]
- mitotic nuclear division [TAS]
- mitotic sister chromatid segregation [TAS]
- mitotic spindle organization [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RPL23A
L23A, MDA20
ribosomal protein L23a
GO Process (14)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- RNA metabolic process [TAS]
- SRP-dependent cotranslational protein targeting to membrane [TAS]
- cell proliferation [IMP]
- cellular protein metabolic process [TAS]
- gene expression [TAS]
- mRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [TAS]
- translation [NAS, TAS]
- translational elongation [TAS]
- translational initiation [TAS]
- translational termination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
- viral transcription [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]
Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]
Quantitative Score
- 20.63554337 [HGSCore]
Throughput
- High Throughput
Additional Notes
- Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11
Curated By
- BioGRID