An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.

Marcon E, Ni Z, Pu S, Turinsky AL, Trimble SS, Olsen JB, Silverman-Gavrila R, Silverman-Gavrila L, Phanse S, Guo H, Zhong G, Guo X, Young P, Bailey S, Roudeva D, Zhao D, Hewel J, Li J, Graeslund S, Paduch M, Kossiakoff AA, Lupien M, Emili A, Wodak SJ, Greenblatt J

Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]

Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]

Quantitative Score

17.84613557 [HGSCore]

Throughput

High Throughput

Additional Notes

Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11

Curated By

BioGRID

Interaction Summary

SMYD3

Gene Ontology Molecular Function

histone-lysine N-methyltransferase activity [ISS]

NME1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]GTP binding [IDA]deoxyribonuclease activity [IDA]identical protein binding [IPI]magnesium ion binding [IDA]nucleoside diphosphate kinase activity [IDA]poly(A) RNA binding [IDA]protein binding [IPI]ribosomal small subunit binding [IPI]

Gene Ontology Cellular Component

Affinity Capture-MS