An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.

Marcon E, Ni Z, Pu S, Turinsky AL, Trimble SS, Olsen JB, Silverman-Gavrila R, Silverman-Gavrila L, Phanse S, Guo H, Zhong G, Guo X, Young P, Bailey S, Roudeva D, Zhao D, Hewel J, Li J, Graeslund S, Paduch M, Kossiakoff AA, Lupien M, Emili A, Wodak SJ, Greenblatt J

Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]

Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]

Quantitative Score

16.68385396 [HGSCore]

Throughput

High Throughput

Additional Notes

Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SSBP1 RPA1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Antonicka H (2020)	High	1	BioGRID	2859572

Curated By

BioGRID

Interaction Summary

RPA1

Gene Ontology Biological Process

Gene Ontology Molecular Function

damaged DNA binding [IDA]protein binding [IPI]single-stranded DNA binding [IDA]

Gene Ontology Cellular Component

SSBP1