F2
Gene Ontology Biological Process
- blood coagulation [TAS]
- blood coagulation, intrinsic pathway [TAS]
- cell surface receptor signaling pathway [IDA]
- cellular protein metabolic process [TAS]
- cytosolic calcium ion homeostasis [IDA]
- fibrinolysis [IDA]
- leukocyte migration [TAS]
- multicellular organismal development [TAS]
- negative regulation of astrocyte differentiation [IDA]
- negative regulation of fibrinolysis [TAS]
- negative regulation of platelet activation [TAS]
- negative regulation of proteolysis [IDA]
- peptidyl-glutamic acid carboxylation [TAS]
- platelet activation [IDA, TAS]
- positive regulation of blood coagulation [IDA]
- positive regulation of collagen biosynthetic process [IDA]
- positive regulation of phospholipase C-activating G-protein coupled receptor signaling pathway [IDA]
- positive regulation of protein phosphorylation [IDA]
- positive regulation of reactive oxygen species metabolic process [IDA]
- positive regulation of release of sequestered calcium ion into cytosol [IDA]
- post-translational protein modification [TAS]
- proteolysis [TAS]
- regulation of blood coagulation [TAS]
- response to wounding [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
F10
Gene Ontology Biological Process
- blood coagulation [IC, TAS]
- blood coagulation, extrinsic pathway [TAS]
- blood coagulation, intrinsic pathway [TAS]
- cellular protein metabolic process [TAS]
- peptidyl-glutamic acid carboxylation [TAS]
- positive regulation of cell migration [TAS]
- positive regulation of protein kinase B signaling [IDA]
- post-translational protein modification [TAS]
- proteolysis [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]
Quantitative Score
- 16.52797374 [HGSCore]
Throughput
- High Throughput
Additional Notes
- Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| F10 F2 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID