BAIT
MED7
ARC34, CRSP33, CRSP9
mediator complex subunit 7
GO Process (3)
GO Function (2)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
APOA2
Apo-AII, ApoA-II, apoAII
apolipoprotein A-II
GO Process (34)
GO Function (13)
GO Component (10)
Gene Ontology Biological Process
- cellular lipid metabolic process [TAS]
- cholesterol efflux [IDA]
- cholesterol homeostasis [IDA]
- diacylglycerol catabolic process [IDA]
- high-density lipoprotein particle assembly [IDA]
- high-density lipoprotein particle clearance [IDA]
- high-density lipoprotein particle remodeling [IDA]
- lipoprotein metabolic process [TAS]
- low-density lipoprotein particle remodeling [IDA]
- negative regulation of cholesterol import [IDA]
- negative regulation of cholesterol transport [IMP]
- negative regulation of cholesterol transporter activity [IDA]
- negative regulation of cytokine secretion involved in immune response [IDA]
- negative regulation of lipase activity [IDA]
- negative regulation of lipid catabolic process [IDA]
- negative regulation of very-low-density lipoprotein particle remodeling [IDA]
- peptidyl-methionine modification [IDA]
- phosphatidylcholine biosynthetic process [IDA]
- phospholipid catabolic process [IDA]
- phospholipid efflux [IDA]
- phototransduction, visible light [TAS]
- positive regulation of catalytic activity [IDA]
- positive regulation of cholesterol esterification [IDA]
- positive regulation of interleukin-8 biosynthetic process [IDA]
- positive regulation of lipid catabolic process [IDA]
- protein folding [IDA]
- protein oxidation [IDA]
- regulation of protein stability [IDA]
- response to glucose [IDA]
- retinoid metabolic process [TAS]
- reverse cholesterol transport [IDA]
- small molecule metabolic process [TAS]
- triglyceride metabolic process [TAS]
- triglyceride-rich lipoprotein particle remodeling [IDA]
Gene Ontology Molecular Function- apolipoprotein receptor binding [IPI]
- cholesterol binding [IDA]
- cholesterol transporter activity [IDA]
- high-density lipoprotein particle receptor binding [IPI]
- lipase inhibitor activity [IDA]
- lipid binding [IDA]
- lipid transporter activity [IDA]
- phosphatidylcholine binding [IDA]
- phosphatidylcholine-sterol O-acyltransferase activator activity [IDA]
- phospholipid binding [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA]
- apolipoprotein receptor binding [IPI]
- cholesterol binding [IDA]
- cholesterol transporter activity [IDA]
- high-density lipoprotein particle receptor binding [IPI]
- lipase inhibitor activity [IDA]
- lipid binding [IDA]
- lipid transporter activity [IDA]
- phosphatidylcholine binding [IDA]
- phosphatidylcholine-sterol O-acyltransferase activator activity [IDA]
- phospholipid binding [IDA]
- protein binding [IPI]
- protein heterodimerization activity [IPI]
- protein homodimerization activity [IDA]
Gene Ontology Cellular Component
- blood microparticle [IDA]
- chylomicron [IDA]
- cytosol [TAS]
- early endosome [TAS]
- endoplasmic reticulum lumen [TAS]
- extracellular region [NAS, TAS]
- extracellular vesicular exosome [IDA]
- high-density lipoprotein particle [IDA]
- spherical high-density lipoprotein particle [IDA]
- very-low-density lipoprotein particle [IDA]
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]
Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]
Quantitative Score
- 16.06689447 [HGSCore]
Throughput
- High Throughput
Additional Notes
- Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11
Curated By
- BioGRID