BAIT
PIP4K2A
PI5P4KA, PIP5K2A, PIP5KII-alpha, PIP5KIIA, PIPK, RP11-301N24.1
phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
GO Process (4)
GO Function (1)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
KNOP1
101F10.1, C16orf88, FAM191A, TSG118
lysine-rich nucleolar protein 1
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]
Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]
Quantitative Score
- 15.6546886 [HGSCore]
Throughput
- High Throughput
Additional Notes
- Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11
Curated By
- BioGRID