BAIT
HTRA2
OMI, PARK13, PRSS25
HtrA serine peptidase 2
GO Process (19)
GO Function (5)
GO Component (11)
Gene Ontology Biological Process
- cellular protein catabolic process [IDA]
- cellular response to growth factor stimulus [IMP]
- cellular response to heat [IDA]
- cellular response to interferon-beta [IDA]
- cellular response to oxidative stress [NAS]
- cellular response to retinoic acid [IDA]
- execution phase of apoptosis [TAS]
- intrinsic apoptotic signaling pathway in response to DNA damage [IMP]
- negative regulation of cell cycle [TAS]
- negative regulation of neuron death [TAS]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [NAS]
- positive regulation of apoptotic process [IMP, TAS]
- positive regulation of cell death [IDA]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway [IMP]
- positive regulation of extrinsic apoptotic signaling pathway in absence of ligand [IMP]
- protein autoprocessing [TAS]
- proteolysis [IMP, TAS]
- regulation of mitochondrion degradation [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
BNIP3
NIP3
BCL2/adenovirus E1B 19kDa interacting protein 3
GO Process (24)
GO Function (5)
GO Component (10)
Gene Ontology Biological Process
- apoptotic process [IPI]
- cell death [ISS]
- cellular response to cobalt ion [IMP]
- cellular response to hypoxia [IMP]
- cellular response to mechanical stimulus [IEP]
- defense response to virus [IDA]
- granzyme-mediated apoptotic signaling pathway [IDA]
- intrinsic apoptotic signaling pathway in response to hypoxia [IMP]
- mitochondrial fragmentation involved in apoptotic process [IDA]
- mitochondrial outer membrane permeabilization [IDA]
- mitochondrial protein catabolic process [IMP]
- negative regulation of apoptotic process [TAS]
- negative regulation of membrane potential [IDA]
- negative regulation of mitochondrial fusion [IDA]
- neuron apoptotic process [ISS]
- positive regulation of apoptotic process [IDA]
- positive regulation of autophagy [TAS]
- positive regulation of mitochondrial fission [IDA]
- positive regulation of programmed cell death [IDA]
- positive regulation of protein complex disassembly [IDA]
- positive regulation of release of cytochrome c from mitochondria [IDA]
- reactive oxygen species metabolic process [IDA]
- regulation of mitochondrial membrane permeability [IDA]
- response to hypoxia [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]
Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]
Quantitative Score
- 13.56478431 [HGSCore]
Throughput
- High Throughput
Additional Notes
- Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11
Curated By
- BioGRID