BAIT
NCBP2
CBC2, CBP20, NIP1, PIG55
nuclear cap binding protein subunit 2, 20kDa
GO Process (21)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- 7-methylguanosine mRNA capping [IDA, TAS]
- RNA metabolic process [TAS]
- RNA splicing [ISS, TAS]
- gene expression [TAS]
- histone mRNA metabolic process [TAS]
- mRNA 3'-end processing [TAS]
- mRNA cis splicing, via spliceosome [IDA]
- mRNA export from nucleus [TAS]
- mRNA metabolic process [TAS]
- mRNA splicing, via spliceosome [TAS]
- ncRNA metabolic process [TAS]
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay [IDA, IMP, TAS]
- positive regulation of RNA export from nucleus [ISS]
- positive regulation of viral transcription [TAS]
- regulation of translational initiation [IDA]
- snRNA export from nucleus [ISS, NAS]
- spliceosomal snRNP assembly [TAS]
- termination of RNA polymerase II transcription [TAS]
- transcription elongation from RNA polymerase II promoter [TAS]
- transcription from RNA polymerase II promoter [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
KPNA1
IPOA5, NPI-1, RCH2, SRP1
karyopherin alpha 1 (importin alpha 5)
GO Process (10)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- NLS-bearing protein import into nucleus [TAS]
- apoptotic DNA fragmentation [TAS]
- apoptotic process [TAS]
- cellular component disassembly involved in execution phase of apoptosis [TAS]
- cytokine-mediated signaling pathway [TAS]
- intracellular transport of virus [TAS]
- positive regulation of protein import into nucleus [ISS]
- regulation of DNA recombination [TAS]
- viral life cycle [TAS]
- viral process [TAS]
Gene Ontology Molecular Function
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]
Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]
Quantitative Score
- 13.39205673 [HGSCore]
Throughput
- High Throughput
Additional Notes
- Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11
Curated By
- BioGRID