BAIT
CSNK2B
CK2B, CK2N, CSK2B, G5A, DADB-127H9.2
casein kinase 2, beta polypeptide
GO Process (13)
GO Function (7)
GO Component (7)
Gene Ontology Biological Process
- adiponectin-activated signaling pathway [IDA]
- axon guidance [TAS]
- cellular protein complex assembly [NAS]
- endothelial tube morphogenesis [IMP]
- mitotic cell cycle [TAS]
- negative regulation of blood vessel endothelial cell migration [IDA]
- negative regulation of cell proliferation [TAS]
- positive regulation of activin receptor signaling pathway [IMP]
- positive regulation of pathway-restricted SMAD protein phosphorylation [IDA]
- protein phosphorylation [TAS]
- regulation of DNA binding [NAS]
- regulation of protein kinase activity [NAS]
- signal transduction [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
CTCF
MRD21
CCCTC-binding factor (zinc finger protein)
GO Process (8)
GO Function (9)
GO Component (5)
Gene Ontology Biological Process
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- nucleosome positioning [IDA]
- positive regulation of gene expression [IMP]
- positive regulation of transcription, DNA-templated [IDA, IMP]
- regulation of centromeric sister chromatid cohesion [NAS]
- regulation of gene expression, epigenetic [IMP]
- regulation of molecular function, epigenetic [IMP]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- chromatin insulator sequence binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription corepressor activity [TAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [TAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]
- chromatin insulator sequence binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [IDA]
- transcription corepressor activity [TAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [TAS]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]
Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]
Quantitative Score
- 12.50124775 [HGSCore]
Throughput
- High Throughput
Additional Notes
- Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11
Curated By
- BioGRID