YBX1
Gene Ontology Biological Process
Gene Ontology Molecular Function- DNA binding [IDA, TAS]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- double-stranded DNA binding [TAS]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- DNA binding [IDA, TAS]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- double-stranded DNA binding [TAS]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
Gene Ontology Cellular Component
- CRD-mediated mRNA stability complex [IDA]
- U12-type spliceosomal complex [IDA]
- cytoplasm [IDA]
- cytoplasmic stress granule [IDA]
- extracellular vesicular exosome [IDA]
- histone pre-mRNA 3'end processing complex [ISS]
- intracellular membrane-bounded organelle [IDA]
- nuclear membrane [IDA]
- nucleoplasm [TAS]
- nucleus [TAS]
- ribonucleoprotein complex [IDA]
SRSF9
Gene Ontology Biological Process
- RNA splicing [TAS]
- gene expression [TAS]
- mRNA 3'-end processing [TAS]
- mRNA export from nucleus [TAS]
- mRNA processing [TAS]
- mRNA splice site selection [TAS]
- mRNA splicing, via spliceosome [TAS]
- negative regulation of mRNA splicing, via spliceosome [IDA]
- termination of RNA polymerase II transcription [TAS]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- nucleolus [IDA]
- nucleoplasm [IDA, TAS]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.
Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]
Quantitative Score
- 11.75205186 [HGSCore]
Throughput
- High Throughput
Additional Notes
- Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SRSF9 YBX1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| YBX1 SRSF9 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID