An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.

Marcon E, Ni Z, Pu S, Turinsky AL, Trimble SS, Olsen JB, Silverman-Gavrila R, Silverman-Gavrila L, Phanse S, Guo H, Zhong G, Guo X, Young P, Bailey S, Roudeva D, Zhao D, Hewel J, Li J, Graeslund S, Paduch M, Kossiakoff AA, Lupien M, Emili A, Wodak SJ, Greenblatt J

Chromatin regulation is driven by multicomponent protein complexes, which form functional modules. Deciphering the components of these modules and their interactions is central to understanding the molecular pathways these proteins are regulating, their functions, and their relation to both normal development and disease. We describe the use of affinity purifications of tagged human proteins coupled with mass spectrometry to generate ... [more]

Cell Rep Jul. 10, 2014; 8(1);297-310 [Pubmed: 24981860]

Quantitative Score

11.2524566 [HGSCore]

Throughput

High Throughput

Additional Notes

Affinity Capture MS carried out to identify high confidence protein interactions with a iHGSCore < 11

Curated By

BioGRID

Interaction Summary

CHERP

Gene Ontology Biological Process

Gene Ontology Molecular Function

ion channel binding [IPI]poly(A) RNA binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

ROCK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein kinase activity [TAS]protein serine/threonine kinase activity [IDA]