An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Role of SUMO activating enzyme in cancer stem cell maintenance and self-renewal.

Du L, Li YJ, Fakih M, Wiatrek RL, Duldulao M, Chen Z, Chu P, Garcia-Aguilar J, Chen Y

Cancer stem cells (CSCs) have key roles in treatment resistance, tumour metastasis and relapse. Using colorectal cancer (CC) cell lines, patient-derived xenograft (PDX) tissues and patient tissues, here we report that CC CSCs, which resist chemoradiation, have higher SUMO activating enzyme (E1) and global SUMOylation levels than non-CSCs. Knockdown of SUMO E1 or SUMO conjugating enzyme (E2) inhibits CC CSC ... [more]

Nat Commun Dec. 28, 2015; 7();12326 [Pubmed: 27465491]

Quantitative Score

6.75 [Custom Score]

Throughput

High Throughput

Additional Notes

Oct1 was affinity purified and associated proteins were identified by mass spectrometry. The method of scoring used was not indicated, but scoring was presumably performed by the Proteome Discoverer 1.4 software. Higher scores presumably indicate higher confidence in the interaction.

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
HNRNPH1 POU2F1	Affinity Capture-RNA Affinity Capture-RNA An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.	Uren PJ (2016)	High	-	BioGRID	2872480

Curated By

BioGRID

Interaction Summary

POU2F1

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]sequence-specific DNA binding [IDA]

Gene Ontology Cellular Component

HNRNPH1