Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Oxidized low-density lipoprotein inhibits the degradation of cyclophilin A via the lysosome in vascular smooth muscle cells.

Su Z, Lin M, Su Y, Li J, Lin R, Wu M, Wang X, Huang L, Chen Y, Shu X, Liang S, Zhang H, Huang T, Nie R, Wang J, Xie S

Cyclophilin A (CyPA) plays an important role in the progression of atherosclerosis. Additionally, it has been reported that lysosomal function is markedly impaired in atherosclerosis induced by oxidized low-density lipoprotein (ox-LDL). As the CyPA degradation pathway remains to be elucidated, we aimed to uncover the role of lysosomes and ox-LDL in the degradation of CyPA.We exploited RNA interference (RNAi) in ... [more]

Am J Transl Res Aug. 11, 2020; 12(7);3964-3973 [Pubmed: 32774749]

Throughput

  • Low Throughput

Curated By

  • BioGRID