BAIT

PMS1

L000001457, YNL082W
ATP-binding protein required for mismatch repair; required for both mitosis and meiosis; functions as a heterodimer with Mlh1p; binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL
Saccharomyces cerevisiae (S288c)
PREY

MLH3

mismatch repair protein MLH3, YPL164C
Protein involved in DNA mismatch repair and meiotic recombination; involved in crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability
GO Process (3)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Exo1 recruits Cdc5 polo kinase to MutL? to ensure efficient meiotic crossover formation.

Sanchez A, Adam C, Rauh F, Duroc Y, Ranjha L, Lombard B, Mu X, Wintrebert M, Loew D, Guarne A, Gnan S, Chen CL, Keeney S, Cejka P, Guerois R, Klein F, Charbonnier JB, Borde V

Crossovers generated during the repair of programmed meiotic double-strand breaks must be tightly regulated to promote accurate homolog segregation without deleterious outcomes, such as aneuploidy. The Mlh1-Mlh3 (MutL?) endonuclease complex is critical for crossover resolution, which involves mechanistically unclear interplay between MutL? and Exo1 and polo kinase Cdc5. Using budding yeast to gain temporal and genetic traction on crossover regulation, ... [more]

Proc Natl Acad Sci U S A Dec. 01, 2019; 117(48);30577-30588 [Pubmed: 33199619]

Throughput

  • Low Throughput

Additional Notes

  • Figure 3

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MLH3 PMS1
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low/High-BioGRID
2953231
PMS1 MLH3
Phenotypic Enhancement
Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Low-BioGRID
1521624
PMS1 MLH3
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Low-BioGRID
800153

Curated By

  • BioGRID