BAIT

MLH3

mismatch repair protein MLH3, YPL164C
Protein involved in DNA mismatch repair and meiotic recombination; involved in crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability
GO Process (3)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

ADE3

trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase ADE3, L000000033, YGR204W
Cytoplasmic trifunctional enzyme C1-tetrahydrofolate synthase; involved in single carbon metabolism and required for biosynthesis of purines, thymidylate, methionine, and histidine; null mutation causes auxotrophy for adenine and histidine
Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Exo1 recruits Cdc5 polo kinase to MutL? to ensure efficient meiotic crossover formation.

Sanchez A, Adam C, Rauh F, Duroc Y, Ranjha L, Lombard B, Mu X, Wintrebert M, Loew D, Guarne A, Gnan S, Chen CL, Keeney S, Cejka P, Guerois R, Klein F, Charbonnier JB, Borde V

Crossovers generated during the repair of programmed meiotic double-strand breaks must be tightly regulated to promote accurate homolog segregation without deleterious outcomes, such as aneuploidy. The Mlh1-Mlh3 (MutL?) endonuclease complex is critical for crossover resolution, which involves mechanistically unclear interplay between MutL? and Exo1 and polo kinase Cdc5. Using budding yeast to gain temporal and genetic traction on crossover regulation, ... [more]

Proc Natl Acad Sci U S A Dec. 01, 2019; 117(48);30577-30588 [Pubmed: 33199619]

Throughput

  • High Throughput

Additional Notes

  • Dataset S3

Curated By

  • BioGRID