Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Cdh1-APC Regulates Protein Synthesis and Stress Granules in Neurons through an FMRP-Dependent Mechanism.

Valdez-Sinon AN, Lai A, Shi L, Lancaster CL, Gokhale A, Faundez V, Bassell GJ

Maintaining a balance between protein degradation and protein synthesis is necessary for neurodevelopment. Although the E3 ubiquitin ligase anaphase promoting complex and its regulatory subunit Cdh1 (Cdh1-APC) has been shown to regulate learning and memory, the underlying mechanisms are unclear. Here, we have identified a role of Cdh1-APC as a regulator of protein synthesis in neurons. Proteomic profiling revealed that ... [more]

iScience May. 22, 2020; 23(5);101132 [Pubmed: 32434143]

Quantitative Score

  • 18.0 [Custom Score]

Throughput

  • High Throughput

Additional Notes

  • FLAG-tagged Cdh1 was affinity purified and associated proteins were identified by mass spectrometry. Scoring type was unclear, but higher values indicate higher confidence in the interaction. High confidence scores for FLAG-CDH1-transfected cells with no competing peptide (18) are reported here. Negative control scores were those for non-transfected cells (0), non-transfected cells with competing FLAG peptide (0) and FLAG-CDH1-transfected cells with competing FLAG peptide (0).

Curated By

  • BioGRID