An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The RING Domain of RING Finger 12 Efficiently Builds Degradative Ubiquitin Chains.

Middleton AJ, Zhu J, Day CL

RNF12 is a widely expressed ubiquitin E3 ligase that is required for X-chromosome inactivation, regulation of LIM-domain containing transcription factors, and TGF-? signaling. A RING domain at the C terminus of RNF12 is important for its E3 ligase activity, and mutations in the RING domain are associated with X-linked intellectual disability. Here we have characterized ubiquitin transfer by RNF12, and ... [more]

J Mol Biol Dec. 12, 2019; 432(13);3790-3801 [Pubmed: 32416094]

Throughput

Low Throughput

Additional Notes

E2:UBE gene

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RLIM UBE2K	Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.	Middleton AJ (2015)	Low	-	BioGRID	2484945

Curated By

BioGRID

Interaction Summary

RLIM

Gene Ontology Biological Process

Gene Ontology Molecular Function

transcription corepressor activity [NAS]ubiquitin-protein transferase activity [ISS]

Gene Ontology Cellular Component

UBE2K

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]small conjugating protein transferase activity [IBA]ubiquitin protein ligase activity [IBA]ubiquitin protein ligase binding [IPI]ubiquitin-protein transferase activity [IDA]ubiquitin-ubiquitin ligase activity [IDA]