An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Cell stemness is maintained upon concurrent expression of RB and the mitochondrial ribosomal protein S18-2.

Mushtaq M, Kovalevska L, Darekar S, Abramsson A, Zetterberg H, Kashuba V, Klein G, Arsenian-Henriksson M, Kashuba E

Stemness encompasses the capability of a cell for self-renewal and differentiation. The stem cell maintains a balance between proliferation, quiescence, and regeneration via interactions with the microenvironment. Previously, we showed that ectopic expression of the mitochondrial ribosomal protein S18-2 (MRPS18-2) led to immortalization of primary fibroblasts, accompanied by induction of an embryonic stem cell (ESC) phenotype. Moreover, we demonstrated interaction ... [more]

Proc Natl Acad Sci U S A Dec. 07, 2019; 117(27);15673-15683 [Pubmed: 32571933]

Throughput

Low Throughput

Additional Notes

Source of MRPS18B not clear

Curated By

BioGRID

Interaction Summary

MRPS18B

Gene Ontology Biological Process

Gene Ontology Molecular Function

structural constituent of ribosome [NAS]

Gene Ontology Cellular Component

PHB2