BAIT
RNF5
RING5, RMA1, DAAP-218M18.2
ring finger protein 5, E3 ubiquitin protein ligase
GO Process (4)
GO Function (2)
GO Component (0)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Homo sapiens
PREY
TMEM33
1600019D15Rik, SHINC3
transmembrane protein 33
GO Process (0)
GO Function (0)
GO Component (2)
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Interaction mapping of endoplasmic reticulum ubiquitin ligases identifies modulators of innate immune signalling.
Ubiquitin ligases (E3s) embedded in the endoplasmic reticulum (ER) membrane regulate essential cellular activities including protein quality control, calcium flux, and sterol homeostasis. At least 25 different, transmembrane domain (TMD)-containing E3s are predicted to be ER-localised, but for most their organisation and cellular roles remain poorly defined. Using a comparative proteomic workflow, we mapped over 450 protein-protein interactions for 21 ... [more]
Elife Dec. 02, 2019; 9(); [Pubmed: 32614325]
Throughput
- High Throughput
Curated By
- BioGRID