BAIT

SMAD4

DPC4, JIP, MADH4, MYHRS
SMAD family member 4
GO Process (24)
GO Function (14)
GO Component (9)
Homo sapiens

Co-localization

Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.

Publication

The evolutionarily conserved deubiquitinase UBH1/UCH-L1 augments DAF7/TGF-? signaling, inhibits dauer larva formation, and enhances lung tumorigenesis.

Nagata A, Itoh F, Sasho A, Sugita K, Suzuki R, Hinata H, Shimoda Y, Suzuki E, Maemoto Y, Inagawa T, Fujikawa Y, Ikeda E, Fujii C, Inoue H

Modification of the transforming growth factor ? (TGF-?) signaling components by (de)ubiquitination is emerging as a key regulatory mechanism that controls cell signaling responses in health and disease. Here, we show that the deubiquitinating enzyme UBH-1 in Caenorhabditis elegans and its human homolog, ubiquitin C-terminal hydrolase-L1 (UCH-L1), stimulate DAF-7/TGF-? signaling, suggesting that this mode of regulation of TGF-? signaling is ... [more]

J Biol Chem Dec. 03, 2019; 295(27);9105-9120 [Pubmed: 32371398]

Throughput

  • Low Throughput

Additional Notes

  • Proximity Ligation Assay
  • Source of SMAD4 not clear

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UCHL1 SMAD4
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
2954492

Curated By

  • BioGRID