BAIT

RPS13

RPS13B, RPS13C, ribosomal 40S subunit protein S13, YS15, S27a, S15, S13, L000002900, L000002655, YDR064W

Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S13 and bacterial S15

GO Process (2)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

cytoplasmic translation [IC]

maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) [IMP]

Gene Ontology Molecular Function

small ribosomal subunit rRNA binding [IDA]
structural constituent of ribosome [IDA]

Gene Ontology Cellular Component

90S preribosome [IDA]

cytosolic small ribosomal subunit [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

MIS1

trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase MIS1, L000001115, YBR084W

Mitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase

GO Process (2)

GO Function (3)

GO Component (1)

Gene Ontology Biological Process

folic acid biosynthetic process [IDA]

nucleobase-containing compound metabolic process [IMP]

Gene Ontology Molecular Function

formate-tetrahydrofolate ligase activity [IDA]
methenyltetrahydrofolate cyclohydrolase activity [IDA]
methylenetetrahydrofolate dehydrogenase (NADP+) activity [IDA]

Gene Ontology Cellular Component

mitochondrion [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Tsr4 and Nap1, two novel members of the ribosomal protein chaperOME.

Roessler I, Embacher J, Pillet B, Murat G, Liesinger L, Hafner J, Unterluggauer JJ, Birner-Gruenberger R, Kressler D, Pertschy B

Dedicated chaperones protect newly synthesized ribosomal proteins (r-proteins) from aggregation and accompany them on their way to assembly into nascent ribosomes. Currently, only nine of the ?80 eukaryotic r-proteins are known to be guarded by such chaperones. In search of new dedicated r-protein chaperones, we performed a tandem-affinity purification based screen and looked for factors co-enriched with individual small subunit ... [more]

Nucleic Acids Res. Dec. 26, 2018; 47(13);6984-7002 [Pubmed: 31062022]

Throughput

High Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
MIS1 RPS13	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Michaelis AC (2023)	High	2	BioGRID	3602136

Curated By

BioGRID

Interaction Summary

RPS13

Gene Ontology Biological Process

Gene Ontology Molecular Function

small ribosomal subunit rRNA binding [IDA]structural constituent of ribosome [IDA]

Gene Ontology Cellular Component

MIS1

Gene Ontology Biological Process

Gene Ontology Molecular Function

formate-tetrahydrofolate ligase activity [IDA]methenyltetrahydrofolate cyclohydrolase activity [IDA]methylenetetrahydrofolate dehydrogenase (NADP+) activity [IDA]

Gene Ontology Cellular Component

Affinity Capture-MS

Publication

Tsr4 and Nap1, two novel members of the ribosomal protein chaperOME.

Throughput

Related interactions

Curated By

small ribosomal subunit rRNA binding [IDA]
structural constituent of ribosome [IDA]

formate-tetrahydrofolate ligase activity [IDA]
methenyltetrahydrofolate cyclohydrolase activity [IDA]
methylenetetrahydrofolate dehydrogenase (NADP+) activity [IDA]