BAIT

PMAIP1

APR, NOXA
phorbol-12-myristate-13-acetate-induced protein 1
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage.

Albert MC, Brinkmann K, Pokrzywa W, Guenther SD, Kroenke M, Hoppe T, Kashkar H

The BH3-only protein NOXA is a regulator of mitochondrial apoptosis by specifically antagonizing the anti-apoptotic protein MCL-1. Here we show that the E3 ubiquitin ligase CHIP controls NOXA stability after DNA damage. Our findings reveal that CHIP and MCL-1 are binding partners of NOXA and differentially define the fate of NOXA. Whereas NOXA is initially targeted to mitochondria upon MCL-1-binding, ... [more]

Cell Death Dis Dec. 10, 2019; 11(9);740 [Pubmed: 32913203]

Throughput

  • High Throughput

Curated By

  • BioGRID