BAIT

PMAIP1

APR, NOXA
phorbol-12-myristate-13-acetate-induced protein 1
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage.

Albert MC, Brinkmann K, Pokrzywa W, Guenther SD, Kroenke M, Hoppe T, Kashkar H

The BH3-only protein NOXA is a regulator of mitochondrial apoptosis by specifically antagonizing the anti-apoptotic protein MCL-1. Here we show that the E3 ubiquitin ligase CHIP controls NOXA stability after DNA damage. Our findings reveal that CHIP and MCL-1 are binding partners of NOXA and differentially define the fate of NOXA. Whereas NOXA is initially targeted to mitochondria upon MCL-1-binding, ... [more]

Cell Death Dis Dec. 10, 2019; 11(9);740 [Pubmed: 32913203]

Throughput

  • High Throughput

Additional Notes

  • E2: UbcH5a/b

Curated By

  • BioGRID