An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Lysine-222 succinylation reduces lysosomal degradation of lactate dehydrogenase a and is increased in gastric cancer.

Li X, Zhang C, Zhao T, Su Z, Li M, Hu J, Wen J, Shen J, Wang C, Pan J, Mu X, Ling T, Li Y, Wen H, Zhang X, You Q

Lysine succinylation is an emerging posttranslational modification that has garnered increased attention recently, but its role in gastric cancer (GC) remains underexplored.Proteomic quantification of lysine succinylation was performed in human GC tissues and adjacent normal tissues by mass spectrometry. The mRNA and protein levels of lactate dehydrogenase A (LDHA) in GC and adjacent normal tissues were analyzed by qRT-PCR and ... [more]

J Exp Clin Cancer Res Aug. 28, 2020; 39(1);172 [Pubmed: 32859246]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CPT1A

Gene Ontology Biological Process

Gene Ontology Molecular Function

carnitine O-palmitoyltransferase activity [IDA]

Gene Ontology Cellular Component

LDHA