BAIT

JAK2

JTK10, THCYT3
Janus kinase 2
GO Process (49)
GO Function (12)
GO Component (7)

Gene Ontology Biological Process

Homo sapiens
PREY

PPP2R1B

PP2A-Abeta, PR65B
protein phosphatase 2, regulatory subunit A, beta
GO Process (2)
GO Function (1)
GO Component (2)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Involvement of protein phosphatase 2A in the interleukin-3-stimulated Jak2-Stat5 signaling pathway.

Yokoyama N, Reich NC, Miller WT

In this study, we report that the tyrosine kinase, Janus kinase 2 (Jak2), associates with the serine/threonine protein phosphatase 2A (PP2A) in 32Dcl3 myeloid progenitor cells. The association between Jak2 and PP2A transiently increases following interleukin-3 (IL-3) stimulation and activation of Jak2. The catalytic subunit of PP2A is tyrosine phosphorylated by Jak2 in vitro and in vivo, resulting in inhibition ... [more]

J. Interferon Cytokine Res. Jun. 01, 2001; 21(6);369-78 [Pubmed: 11440634]

Throughput

  • Low Throughput

Curated By

  • BioGRID