BAIT

PCNA

ATLD2
proliferating cell nuclear antigen
Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

TRIM28 functions as the SUMO E3 ligase for PCNA in prevention of transcription induced DNA breaks.

Li M, Xu X, Chang CW, Liu Y

In human cells, the DNA replication factor proliferating cell nuclear antigen (PCNA) can be conjugated to either the small ubiquitinlike modifier SUMO1 or SUMO2, but only SUMO2-conjugated PCNA is induced by transcription to facilitate resolution of transcription-replication conflict (TRC). To date, the SUMO E3 ligase that provides substrate specificity for SUMO2-PCNA conjugation in response to TRC remains unknown. Using a ... [more]

Proc Natl Acad Sci U S A Dec. 22, 2019; 117(38);23588-23596 [Pubmed: 32900933]

Throughput

  • High Throughput

Curated By

  • BioGRID