An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Profilin is required for sustaining efficient intra- and intercellular spreading of Shigella flexneri.

Mimuro H, Suzuki T, Suetsugu S, Miki H, Takenawa T, Sasakawa C

The ability of Shigella to mediate actin-based motility within the host cell is a prominent pathogenic feature of bacillary dysentery. The ability is dependent on the interaction of VirG with neural Wiskott-Aldrich syndrome protein (N-WASP), which in turn mediates recruitment of Arp2/3 complex and several actin-related proteins. In the present study, we show that profilin I is essential to the ... [more]

J. Biol. Chem. Sep. 15, 2000; 275(37);28893-901 [Pubmed: 10867004]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

PFN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

actin binding [IPI]adenyl-nucleotide exchange factor activity [IDA]phosphatidylinositol-4,5-bisphosphate binding [IDA]poly(A) RNA binding [IDA]proline-rich region binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

VIPR1