SLC4A1
Gene Ontology Biological Process
Gene Ontology Molecular Function- anion transmembrane transporter activity [TAS]
- anion:anion antiporter activity [IDA]
- ankyrin binding [IPI]
- bicarbonate transmembrane transporter activity [IDA]
- chloride transmembrane transporter activity [ISS]
- inorganic anion exchanger activity [IDA, TAS]
- protein anchor [TAS]
- protein binding [IPI]
- protein homodimerization activity [IPI]
- anion transmembrane transporter activity [TAS]
- anion:anion antiporter activity [IDA]
- ankyrin binding [IPI]
- bicarbonate transmembrane transporter activity [IDA]
- chloride transmembrane transporter activity [ISS]
- inorganic anion exchanger activity [IDA, TAS]
- protein anchor [TAS]
- protein binding [IPI]
- protein homodimerization activity [IPI]
Gene Ontology Cellular Component
CA4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- anchored component of external side of plasma membrane [IDA]
- apical plasma membrane [IDA]
- basolateral plasma membrane [IDA]
- brush border membrane [IDA]
- cell surface [IDA]
- endoplasmic reticulum-Golgi intermediate compartment [IDA]
- extracellular vesicular exosome [IDA]
- membrane [TAS]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA, TAS]
- rough endoplasmic reticulum [IDA]
- secretory granule membrane [IDA]
- trans-Golgi network [IDA]
- transport vesicle membrane [IDA]
Co-fractionation
Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.
Publication
The extracellular component of a transport metabolon. Extracellular loop 4 of the human AE1 Cl-/HCO3- exchanger binds carbonic anhydrase IV.
Cytosolic carbonic anhydrase II (CAII) and the cytoplasmic C-terminal tails of chloride/bicarbonate anion exchange (AE) proteins associate to form a bicarbonate transport metabolon, which maximizes the bicarbonate transport rate. To determine whether cell surface-anchored carbonic anhydrase IV (CAIV) interacts with AE proteins to accelerate the bicarbonate transport rate, AE1-mediated bicarbonate transport was monitored in transfected HEK293 cells. Expression of the ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SLC4A1 CA4 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID