Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

ISG15 Connects Autophagy and IFN-?-Dependent Control of Toxoplasma gondii Infection in Human Cells.

Bhushan J, Radke JB, Perng YC, Mcallaster M, Lenschow DJ, Virgin HW, Sibley LD

The intracellular protozoan parasite Toxoplasma gondii is capable of infecting most nucleated cells, where it survives in a specially modified compartment called the parasitophorous vacuole (PV). Interferon gamma (IFN-?) is the major cytokine involved in activating cell-autonomous immune responses to inhibit parasite growth within this intracellular niche. In HeLa cells, IFN-? treatment leads to ubiquitination of susceptible parasite strains, recruitment ... [more]

mBio Dec. 06, 2019; 11(5); [Pubmed: 33024031]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ISG15 AHNAK
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
678115

Curated By

  • BioGRID