An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

NDRG2 regulates adherens junction integrity to restrict colitis and tumourigenesis.

Wei M, Ma Y, Shen L, Xu Y, Liu L, Bu X, Guo Z, Qin H, Li Z, Wang Z, Wu K, Yao L, Li J, Zhang J

Paracellular barriers play an important role in the pathogenesis of Inflammatory bowel disease (IBD) and maintain gut homeostasis. N-myc downstream-regulated gene 2 (NDRG2) has been reported to be a tumour suppressor gene and to inhibit colorectal cancer metastasis. However, whether NDRG2 affects colitis initiation and colitis-associated colorectal cancer is unclear.Intestine-specific Ndrg2 deficiency mice (Ndrg2?IEC) were subjected to DSS- or TNBS-induced ... [more]

EBioMedicine Nov. 01, 2020; 61();103068 [Pubmed: 33099085]

Throughput

Low Throughput

Additional Notes

Sources of NDRG2 and SNAIL not clear

Curated By

BioGRID

Interaction Summary

SNAI1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription [IDA]kinase binding [IPI]protein binding [IPI]

Gene Ontology Cellular Component

NDRG2