BAIT

SEMA4D

C9orf164, CD100, M-sema-G, SEMAJ, coll-4, XXyac-YM68G7.1
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
Homo sapiens
PREY

PTPRC

B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200, RP11-553K8.4
protein tyrosine phosphatase, receptor type, C
GO Process (27)
GO Function (4)
GO Component (6)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

CD100 is associated with CD45 at the surface of human T lymphocytes. Role in T cell homotypic adhesion.

Herold C, Elhabazi A, Bismuth G, Bensussan A, Boumsell L

CD100 is a 150-kDa human surface glycoprotein implicated in T cell activation. Using BB18 and BD16 mAbs to two discrete epitopes of the CD100 molecule, we have shown previously that triggering the CD100 molecule through the BB18 epitope is comitogenic with PMA, whereas it lacks a detectable effect on CD2- and CD3-induced PBMC proliferation. Conversely, triggering the CD100 molecule through ... [more]

J. Immunol. Dec. 15, 1996; 157(12);5262-8 [Pubmed: 8955171]

Throughput

  • Low Throughput

Curated By

  • BioGRID