PREY

FYN

SLK, SYN, p59-FYN, RP1-66H14.1
FYN proto-oncogene, Src family tyrosine kinase
GO Process (29)
GO Function (6)
GO Component (5)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Activation of src family kinases in human pre-B cells by IL-7.

Seckinger P, Fougereau M

IL-7 was identified originally as a specific pre-B cell growth factor. We have investigated its signal transduction mechanism by using the human pre-B cell line Nalm-6, and have found that it stimulates tyrosine phosphorylation of various proteins: pp27, pp43, pp54, pp64, pp78, pp90, pp105, and pp120. Antiphosphotyrosine immunoprecipitates from IL-7-stimulated Nalm-6 showed two major proteins of M(r) = 60,000 and ... [more]

J. Immunol. Jul. 01, 1994; 153(1);97-109 [Pubmed: 7515933]

Throughput

  • Low Throughput

Curated By

  • BioGRID