An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The LIM/double zinc-finger motif functions as a protein dimerization domain.

Feuerstein R, Wang X, Song D, Cooke NE, Liebhaber SA

Protein-protein interactions resulting in dimerization and heterodimerization are of central importance in the control of gene expression and cell function. Proteins that share the 52-residue LIM/double zinc-finger domain are involved in a wide range of developmental and cellular controls. Some of these functions have been hypothesized to involve protein dimerization. In the present report we demonstrate, using both in vitro ... [more]

Proc. Natl. Acad. Sci. U.S.A. Oct. 25, 1994; 91(22);10655-9 [Pubmed: 7938009]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CSRP1 CSRP1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Feuerstein R (1994)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

CSRP1

Gene Ontology Biological Process

Gene Ontology Molecular Function

poly(A) RNA binding [IDA]

Gene Ontology Cellular Component